Yes. Quality Biological, Inc.,’s products and/or packaging contains recycled/renewable content, reduce waste, and are safer to human and environmental health.
All Certificates of Analysis can be found here with the product catalog number and lot number.
Product SDS and other information is available on the product’s page.
Quality Biological, Inc., Serum Free Media do not contain L-Glutamine and should be supplemented with L-glutamine to a final concentration suitable to your specific cell line. Most cell lines will require supplementing with L-Glutamine to a final concentration of 2-6 mM prior to use.
When stored properly at -10 to -20° C, sera can be used for up to 48 months. All our basal media stored at +4° C will have a 2-year shelf life from the time of manufacturing. However, DMEM is the only basal medium with a 1-year shelf life, the medium tends to precipitate after 12 months even if refrigerated.
Water has no buffering capacity. It is not unusual for water to range in pH from 2 to 10 units.
There are two commonly implemented methods, “The Weaning or Step-Down Method” and “The Quick Method.” Description for these two methods can be found here.
We are committed to providing the very best custom manufacturing service and we are pleased to assist with both small and large volume requirements. If the product of interest must be sterile filtered, our minimum volume requirement is 25 Liters, whereas for products that can be autoclaved, our minimum volume is only 5 Liters. Custom products can be packaged into 1 mL to 20 Liters size containers.
Our Contract Manufacturing Information Request Form can be found here.
Our Custom Media Information Request Form can be found here.
Water has no buffering capacity. It is not unusual for water to range in pH from 2 to 10 units.
SOC medium is SOB medium supplemented with 20 mM glucose.
The difference between these two bacteriological media is their respective sodium chloride concentrations. Lennox L broth contains 5.0 g/L NaCl and Luria-Bertani broth contains 10.0 g/L.
Regardless of whether our product is TE, pH 8.0 or TE, pH 7.4, the concentrations of Tris (T) and EDTA (E) are 10 mM and 1 mM respectively.
Our Prehybridization/Hybridization Buffer is qualified for DNA applications only (e.g. Southern Blotting). For RNA applications (e.g. Northern Blotting), we recommend our Hybridization Buffer for Nylon Membranes. Catalog #351-059-101.
Our DEPC (Diethyl pyrocarbonate) elimination process is proprietary.
See the below table.
| Components | RNA Gel Loading Solutions, 10X | DNA Gel Loading Solution, 5X | SDS Protein Gel Loading Solution, 2X |
|---|---|---|---|
| Bromophenol Blue | 0.25% (w/v) | 0.25% (w/v) | 0.2% (w/v) |
| Xylene cyanole | 0.25% (w/v) | 0.25% (w/v) | – |
| EDTA | 1 mM | – | – |
| Glycerol | 50% (v/v) | 30% (v/v) | 20% |
| Tris base | – | – | 100 mM, pH 6.8 |
| SDS | – | – | 4.0% (w/v) |
The dyes migrate as follows:
In 1% agarose TAE buffer, Xylene cyanole-4160 bp, bromophenol blue-370 bp
In 1% agarose TBE buffer, Xylene cyanole-3030 bp, bromophenol blue-220 bp
MOPS 0.2 M, Sodium acetate-3H2O 0.08 M, Disodium EDTA-2H2O 0.01 M, pH adjusted to 7.0.
Formamide, an organic denaturant, is commonly used in nucleic acid applications such as sequencing. It is well known that formamide, even at very low temperatures (e.g., –20 ° C), will break down into formic acid and ammonia. Both by-products are capable of degrading RNA and DNA. Thus, it is prudent to use freshly deionized formamide before adding it to our DNA Gel Loading Solution and/or to our RNA Gel Loading Solution.
Our regular agar plates are supplied in two sizes: 15 X 100mm (standard) and 15 X 150mm but we can manufacture custom orders with other formats. Our minimum custom order requirement is 100 plates. You may specify a fill volume; we typically do 25 and 40mL fills. In most cases, we can deliver a custom order within 5-10 business days.
