Cell Biology FAQ’sCB-1. Should I supplement Quality Biological serum-free media (SFM) with L-glutamine prior to use? If so, what should be the final concentration of L-glutamine?
CB-2. How stable are your sera and basal media?
CB-3. What is the pH of your Cell Culture Grade water?
CB-4. How do I adapt my cells from a serum-containing medium to one of your serum-free media (SFM)?
CB-5. I am interested in your custom cell biology products. What are your minimum requirements for customized reagents?
Molecular Biology FAQ’sMB-1. What is the pH of your DEPC-treated Water and Molecular Biology Grade Water?
MB-2. What is the difference between your SOC and SOB media?
*Omitted from preparation. Add 20mM of MgCl2 prior to use.
MB-3. What is the difference between your Lennox L and Luria-Bertani media?
The difference between these two bacteriological media is their respective sodium chloride concentrations. Lennox L broth contains 5.0 g/L NaCl as opposed to Luria-Bertani which has 10.0 g/L of sodium chloride.
MB-4. What is the formulation of your TE buffers?
Regardless of whether our product is TE, pH 8.0 or TE, pH 7.4, the concentrations of Tris (T) and EDTA (E) are 10mM and 1mM respectively. Click on TIS to access the library of Technical Information Sheets for more information.
MB-5. Is your Prehybridization/Hybridization buffer qualified for RNA?
Our Prehybridization/Hybridization Buffer is qualified for DNA applications only (e.g. Southern Blotting). Click on TIS to access the library of Technical Information Sheets for more information.
For RNA applications (e.g. Northern Blotting), we recommend our Hybridization Buffer for Nylon Membranes. Click on TIS to access the library of Technical Information Sheets for more information.
MB-6. How do you remove DEPC from your DEPC-treated water?
Our DEPC (Diethyl pyrocarbonate) elimination process is proprietary. However, once we remove the DEPC from our DEPC-treated water, it is sterile filtered through 0.1 µm filter into 100 and 1L plastic (PET) bottles. Click on TIS to access the library of Technical Information Sheets for more information.
MB-7. What are the compositions of your RNA, DNA, and SDS-Protein Gel Loading Solutions?
See the table below.
|Components||RNA Gel Loading Solutions, 10X||DNA Gel Loading Solution, 5X||SDS Protein Gel Loading Solution, 2X|
|Bromophenol Blue||0.25% (w/v)||0.25% (w/v)||0.2% (w/v)|
|Xylene cyanole||0.25% (w/v)||0.25% (w/v)||–|
|Glycerol||50% (v/v)||30% (v/v)||20%|
|Tris base||–||–||100 mM, pH 6.8|
MB-8. Where do xylene cyanole (XC) and bromophenol blue (BPB) dyes migrate in agarose gel electrophoresis? Where do these two tracking dyes migrate in a polyacrylamide gel?
Xylene cyanole (XC) and bromophenol blue (BPB) are tracking dyes for gel electrophoresis. According to either “Maniatis” (Sambrook, Fritsch & Maniatis (1989) Molecular Cloning, A laboratory Manual; 2nd Edition., CSHL Press) or FMC, the dyes migrate as follows:
i). Migration in a polyacrylamide gel:
ii). Migration in a denaturing polyacrylamide gel:
iii). Migration in an agarose gel (Source: FMC):
MB-9. What is the formulation of your RNA Gel Buffer (10X MOPS Buffer)?
The formulation is provided in a Technical Information sheet available from Customer Service. Alternatively, it may be viewed on-line.
Click on TIS to access the library of Technical Information Sheets for more information.
MB-10. Why do you not put formamide in your gel loading solutions?
Formamide, an organic denaturant, is commonly used in nucleic acid applications such as sequencing. It is well known that formamide, even at very low temperatures (e.g., –20 ° C), will break down into formic acid and ammonia. Both by-products are capable of degrading RNA and DNA. Thus, it is prudent to use freshly deionized formamide before adding it to our DNA Gel Loading Solution and/or to our RNA Gel Loading Solution. Click on TIS to access the library of Technical Information Sheets for more information.
MB-11. I understand that Quality Biological is in the business of preparing custom agar plates? What is your minimum custom order? What size plates do you use and what are their fill volumes? What is the turn-around time?
Our agar plates are supplied in two sizes: 15 X 100mm (standard) and 15 X 150mm. Our minimum requirement is 100 plates. You may specify a fill volume; we typically do 25 and 40mL fills. In most cases, we can deliver a custom order within 5-10 business days.