Description
ACK Lysing Buffer is used for lysing red blood cells (RBCs) in samples containing white blood cells.
RBCs are permeable to ammonium chloride, which causes cellular swelling and eventual rupture of the cell membrane.
ACK = Ammonium-Chloride-Potassium
| Ammonium Chloride | 155mM |
| Potassium bicarbonate | 10mM |
| EDTA | 0.1mM |
Applications
ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated whole blood, buffy coats, and bone marrow. Commonly performed as part of processing samples for flow cytometry analysis.
Download references citing the use of ACK Lysing Buffer.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
Sterile Filtered
pH: 7.4 ± 0.2
Osmolality: 290±5%mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Harmonization Code: 3822.00.5090
ECCN: EAR99
