RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.
- With 2.0 g/L sodium bicarbonate
- With HEPES buffer
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures.
The original Click formulation was published in 1972 and is a modification of Eagle’s Minimum Essential Medium with Hanks’ salts (HMEM). RPMI 1640: Click;s Medium (1:1) has increased concentrations of many essential amino acids, addition of non-essential amino acids, sodium pyruvate and nucleic acid precursors.
Wilson & Horster used the 1:1 mix of RPMI-1640 and Click’s medium to study the differential response of defined distal nephron epithelia in culture. RPMI-1640 Click’s medium is frequently used for the suspension, washing and culture of T-cells.
Storage Conditions: 2°C to 8°C
Shipping Condition: ambient temperature
pH: 7.0 ± 0.2
Osmolality: 300 ± 5% mOsm/Kg H2O
Harmonization Code: 3821.00.0000
Max Shelf Life: 24 months