DNA Denaturing Solution

ItemSizeList PriceQty
351-013-131 1000 mL $50.36
351-013-151 10 L $472.38

Description

DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting.

  • Contains: 0.5 M NaOH and 1.5 M NaCl
  • Filtered through a 0.22 µm membrane
  • DNase tested

Applications

The Denaturing Solution is a strong base, 0.5 M NaOH with 1.5 M NaCl, that denatures double-stranded DNA, so that it can be efficiently transferred to a nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe.

When utilized in conjunction with a depurination solution, treatment with the denaturing solution results in the hydrolysis of the phosphodiester backbone at the sites of depurination. This allows for a more efficient transfer of large DNA in neutral Southern transfers. Following DNA denaturation, the gel must be neutralized by soaking in DNA Neutralization Solution.

 

Max Shelf Life: 36 months

Storage Condition: 15°C to 30°C

Shipping Condition: ambient temperature

Sterile Filtered

DNase Tested

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

UNSPSC: 41116126

Harmonization Code: 3822.00.5090

ECCN: EAR99

 

Additional information

Product Manufacturer

Quality Biological

Product Documentation

Technical Information Sheet 351-013