GASP is useful for reducing or eliminating bacterial contamination in primary cell cultures. Recommended for use at 10 mL/L
- With L-glutamine, chlortetracycline, penicillin and streptomycin
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.
Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria. This solution contains 25,000 units of penicillin, 25,000 µg/mL of streptomycin, and 25,000 µg/mL of chlortetracycline and 200mM of L-glutamine in physiological saline.
Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. It inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. It is effective against Gram-positive and to a lesser degree Gram-negative bacteria than tetracycline.
GASP contains 25,000 units of penicillin, 25,000 µg/mL of streptomycin, 2,500 µg/mL chlortetracycline and 200 mM of L-glutamine in a physiological saline.
Storage Conditions: -10°C to -20°C
Shipping Condition: on dry ice
Harmonization Code: 3822.00.5090
Max Shelf Life: 24 months