Ham’s F-12 without Hypoxanthine and L-Glutamine

ItemSize List Price Qty
112-153-131 1000 mL $45.48

Description

Compared to other basal media, Ham’s F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Ham’s F-12 contains no proteins or growth factors, and uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH.

  • Without hypoxanthine and L-glutamine

Applications

Ham’s F-12 Nutrient Mixture without Hypoxanthine is useful for serum free clonal growth of Chinese Hamster Ovary (CHO) cells, chick embryos and mouse L-cells. Ham’s F-12 Nutrient Mixture without Hypoxanthine can also be used to culture myeloma cells and hybridomas, as well as primary rat hepatocytes and prostate epithelial cells.

Recommendation

Add L-glutamine prior to use at a final concentration of 1.0 mM.
Supplement 10% FBS because F-12 contains no proteins or growth factors.

 

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

Osmolality: 295±5%mOsm/Kg H20

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3821.00.0000

ECCN: EAR99

Max Shelf Life: 24 months

 

Additional information

Weight 48 oz
Dimensions 4 × 12 × 10 in
Vendor Number

112-153-131

Unit Quantity

1000 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 112-153
Formulation 112-153