HEPES Buffer (1 M), pH 7.3

ItemSize List Price Qty
118-089-721 Pack 4 X 100 mL $372.41


HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research.

  • pH 7.2 – 7.6


HEPES 1M is used as a buffer alternative from sodium bicarbonate for cell culture. HEPES is effective as a buffer at pH 6.8 to 8.2. HEPES is also attributed with characteristics such as: pKa values between 6.0 and 8.0, high solubility membrane impermeability, limited effect on biochemical reactions, very low visible and ultraviolet light absorbance, chemically and enzymatically stable, and easy to prepare.

HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is commonly used as a strong buffering agent.


Dilute 1 M HEPES in the appropriate cell culture medium to a final concentration of 10-25 mM.

Storage Conditions: 15°C to 30°C

Shipping Condition: ambient temperature

Sterile Filtered

Performance Tested

pH: 7.2-7.4

Harmonization Code: 3822.00.5090


Max Shelf Life: 24 months

Additional information

Weight 32 oz
Dimensions 8 × 6 × 6 in
Vendor Number


Unit Quantity

Pack 4 X 100 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 118-089
Technical Information Sheet 118-089