Most media are buffered by a combination of sodium bicarbonate and an atmosphere enriched in CO2. The latter is usually provided through the use of a specially equipped incubator. L-15 was developed at a time when such equipment was not readily available. Dr. Leibovitz developed a buffering system based on free base amino acids, inorganic salts and the substitution of galactose for glucose.
- Without Sodium Bicarbonate
- Designed for cell culture without CO2
- With Phenol Red
- Without L-glutamine
L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been cultivated in this medium.
Storage Conditions: 2°C to 8°C
Shipping Condition: ambient temperature
pH: 7.4 ± 0.3
Osmolality: 315±5%mOsm/Kg H20
Harmonization Code: 3821.00.0000
Max Shelf Life: 24 months