Leibovitz’ L-15 Medium without L-Glutamine

Description

Most media are buffered by a combination of sodium bicarbonate and an atmosphere enriched in CO₂. The latter is usually provided through the use of a specially equipped incubator. L-15 was developed at a time when such equipment was not readily available. Dr. Leibovitz developed a buffering system based on free base amino acids, inorganic salts and the substitution of galactose for glucose.

• Without Sodium Bicarbonate
• Designed for cell culture without CO₂
• With Phenol Red
• Without L-glutamine

Applications

L-15 has been used for the culture of Hep-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been cultivated in this medium.

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.4 ±  0.3

Osmolality: 315±5%mOsm/Kg H₂0

Max Shelf Life: 24 months from date of manufacture

Harmonization Code: 3821.00.0000

ECCN: EAR99