Description
Low EDTA TE (1X), pH 8.0 is molecular biology grade TE buffer with 0.1mM EDTA and is used to store DNA and RNA.
EDTA chelates Mg+2 and other divalent metal ions – (inhibits DNase and RNase to suppress DNA and RNA degradation). Some enzymes, such as Taq polymerase used in PCR, require a certain level of Mg+2 for enzymatic activity. This version of TE buffer, with 10X less EDTA, does not interfere with the Mg+2 in the reactions.
Tris is a buffering agent to keep the solution at a defined pH – The ‘Cl’ or ‘HCl’ indicates the pH was attained using aqueous hydrochloric acid (HCl), since Tris is naturally basic. Tris–EDTA (TE) is routinely used for suspending nucleic acid samples.
- With 0.1 mM EDTA
- Filtered through a 0.1 µm membrane
- DNase, RNase, and Protease tested
Applications
Low EDTA is recommended to re-suspend final purified DNA when DNA is used for downstream PCR reactions.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
Sterile Filtered
pH: 8.0 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Harmonization Code: 3822.00.5090
ECCN: EAR99