McCoy’s 5A Medium with L-Glutamine

ItemSize List Price Qty
112-028-101 500 mL $23.77


McCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

  • With L-glutamine and Phenol Red


McCoy’s 5A Medium originated from Dr. McCoy and his coworkers, as it was centered on the amino acid requirements of Novikoff hepatoma cells in culture. This work involved a medium known as Basal Medium 5A and used to grow normal bone marrow, skin, gingiva, testes, mouse kidney, omentum, adrenal glands, lung, spleen, rat embryos and other tissues. This product also includes Dr. Hsu’s addition of Hanks’ salts to enable use outside a CO2 incubator.

McCoy’s 5A Medium with L-glutamine will support growth of primary mammalian cells derived from the following: normal bone marrow skin, spleen, kidney, lung, rat embryos, and other tissues.


Max Shelf Life: 24 months

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.0±0.2

Osmolality: 290±5% mOsm/Kg H2O

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3821.00.0000




Additional information

Weight 32 oz
Dimensions 3 × 12 × 7 in
Vendor Number


Unit Quantity

500 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 112-028
Formulation 112-028