Medium 199 (E-199) without L-Glutamine and Phenol Red

ItemSize List Price Qty
112-254-101 500 mL $47.71


E199 or Medium 199 with Earle’s Salt is one of the best known media in the history of cell culture. Morgan, Morton and Parker’s Medium 199 was the first really successful attempt to develop a totally defined medium. Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts.

  • Without Phenol Red
  • Without L-glutamine, Hepes


E199 has broad species applicability, particularly for cultivation of non-transformed cells. Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.

E199 has been used for many different types of cells, especially non-transformed cells, and proved particularly useful in polio vaccine production and other virology applications such as vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. QBI offers a variety of E-199 Medium modifications for a range of cell culture applications.


Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

Osmolality: 290±5%mOsm/Kg H20

Max Shelf Life: 24 months from date of manufacture

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3821.00.0000




Additional information

Weight 32 oz
Dimensions 3 × 12 × 7 in
Vendor Number


Unit Quantity

500 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 112-254
Formulation 112-254