Prehybridization Buffer is a general purpose buffer for DNA prehybridization and hybridization procedures. It is particularly useful for procedures involving DNA immobilized on nitrocellulose membrane.
General purpose buffer for all prehybridization and hybridization.
Filtered through a 0.2 µm membrane
Prehybridization Buffer is useful in Southern and Northern blotting protocols to block non-specific DNA interactions and reduce background noise. Prehybridization Buffer is also useful for SDS-PAGE, Gel electrophoresis and Western blotting.
Use 0.1 ml of Prehybridization/Hybridization Buffer per 1 cm2 of filter area. To further reduce nonspecific background, denatured, fragmented salmon sperm DNA at 100 µg/ml may be added to this hybridization buffer.
QBI’s Prehybridization/Hybridization Buffer is qualified for DNA applications only. For RNA applications Hybridization Buffer for Nylon Membrane is recommended. If Prehybridization/Hybridization Buffer gels at low temperature, warm at 30°C – 40°C until the solution clears. This gelling will not affect the performance of the product.