Description
Prehybridization Buffer is a general purpose buffer for DNA prehybridization and hybridization procedures. It is particularly useful for procedures involving DNA immobilized on nitrocellulose membrane.
- General purpose buffer for all prehybridization and hybridization.
- Filtered through a 0.2 µm membrane
- DNase tested
Applications
Prehybridization Buffer is useful in Southern and Northern blotting protocols to block non-specific DNA interactions and reduce background noise. Prehybridization Buffer is also useful for SDS-PAGE, Gel electrophoresis and Western blotting.
Recommendation
Use 0.1 ml of Prehybridization/Hybridization Buffer per 1 cm2 of filter area. To further reduce nonspecific background, denatured, fragmented salmon sperm DNA at 100 µg/ml may be added to this hybridization buffer.
QBI’s Prehybridization/Hybridization Buffer is qualified for DNA applications only. For RNA applications Hybridization Buffer for Nylon Membrane is recommended. If Prehybridization/Hybridization Buffer gels at low temperature, warm at 30°C – 40°C until the solution clears. This gelling will not affect the performance of the product.
Storage Conditions: 25°C
Shipping Condition: ambient temperature
Sterile Filtered
DNase Tested
Harmonization Code: 3822.00.5090
ECCN: EAR99
Max Shelf Life: 36 months
