Prehybridization Buffer

ItemSize List Price Qty
351-077-101 500 mL


Prehybridization Buffer is a general purpose buffer for DNA prehybridization and hybridization procedures. It is particularly useful for procedures involving DNA immobilized on nitrocellulose membrane.

  • General purpose buffer for all prehybridization and hybridization.
  • Filtered through a 0.2 µm membrane
  • DNase tested


Prehybridization Buffer is useful in Southern and Northern blotting protocols to block non-specific DNA interactions and reduce background noise. Prehybridization Buffer is also useful for SDS-PAGE, Gel electrophoresis and Western blotting.


Use 0.1 ml of Prehybridization/Hybridization Buffer per 1 cm2 of filter area. To further reduce nonspecific background, denatured, fragmented salmon sperm DNA at 100 µg/ml may be added to this hybridization buffer.
QBI’s Prehybridization/Hybridization Buffer is qualified for DNA applications only. For RNA applications Hybridization Buffer for Nylon Membrane is recommended. If Prehybridization/Hybridization Buffer gels at low temperature, warm at 30°C – 40°C until the solution clears. This gelling will not affect the performance of the product.


Storage Conditions: 25°C

Shipping Condition: ambient temperature

Sterile Filtered

DNase Tested

Max Shelf Life: 36 months from date of manufacture

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3822.00.5090


Additional information

Weight 32 oz
Dimensions 3 × 12 × 7 in
Vendor Number


Unit Quantity

500 mL

Product Manufacturer

Quality Biological