Description
QBSF-56 is a proprietary serum-free formulation engineered to support human lymphocytes and cell cultures.
In addition, QBSF-56 can be used as an intermediate medium in the process of weaning fastidious cells from growth in a serum-supplemented medium to a low protein serum-free medium. QBSF-56 has also been used successfully to support the proliferation of primary cell cultures.
- Serum-Free
- Contains L-glutamine & Phenol Red
- Endotoxin, mycoplasma, and performance tested
- Sterile Filtered
Applications
QBSF-56 is designed to support human peripheral blood blastogenesis and cell cultures. The following cell lines have been successfully propagated in QBSF-56: SP2/O AG14, SP2 MAI, SP2 SAS, VERO, A 3.01 (infected), SP2 SA3, SP2 SSI, U 937, H9 (HIV infected), and CEM.
The QBSF products have been used successfully in different cell culture applications; investigators are encouraged to explore new uses.
QBSF products used in various applications
- Serum-free culture conditions for cells capable of producing long-term survival in lethally irradiated mice. Brown et al. Stem Cells. 1997;15(3):237-45.
- HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu et al., Stem Cells. 2006 Apr;24(4):876-88.
- Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation. Schuringa et al., J Exp Med. 2004 Sep 6;200(5):623-35.
- Acute Sensitivity of Ph-like Acute Lymphoblastic Leukemia to the SMAC-Mimetic Birinapant. Richmond et al., Cancer Res. 2016 Aug 1;76(15):4579-91.
- Initial CD34+ cell-enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon ex vivo expansion. Andrade et al., J Cell Biochem. 2011 Jul;112(7):1822-31.
- Efficient iPS cell generation from blood using episomes and HDAC inhibitors. Hubbard JJ. et al., J Vis Exp. 2014 Oct 28;(92):e52009.
- Short-term exposure of umbilical cord blood CD34+ cells to granulocyte-macrophage colony-stimulating factor early in culture improves ex vivo expansion of neutrophils. Marturana et al., Cytotherapy. 2011 Mar;13(3):366-77.
- Stromal cell-free conditions favorable for human B lymphopoiesis in culture. Ichii et al., J Immunol Methods. 2010 Jul 31;359(1-2):47-55.
- Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cruz et al. Cytotechnology. 1998 Jan;26(1):59-64.
- Grafts of adenosine-releasing cells suppress seizures in kindling epilepsy. Huber et al. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7611-6.
- Cytosine deaminase adenoviral vector and 5-fluorocytosine selectively reduce breast cancer cells 1 million-fold when they contaminate hematopoietic cells: a potential purging method for autologous transplantation. Garcia-Sanchez et al. Blood. 1998 Jul 15;92(2):672-82.
Storage Conditions: 2°C to 8°C
Shipping Condition: ambient temperature
Sterile Filtered
pH: 7.0± 0.2
Osmolality: 285 ± 3% mOsm/Kg H2O
Harmonization Code: 3821.00.0000
ECCN: EAR99
Max Shelf Life: 24 months
