RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.
- With 2.0 g/L sodium bicarbonate
- Without HEPES buffer
- With stable L-Alanyl-Glutamine (Glutamine XL)
Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life.
The incorporated L-alanine protects the unstable alpha amino acid group of L-glutamine. Aminopeptidase within the cells cleaves the dipeptide, gradually releasing both L-glutamine and L- alanine for use
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.
Common applications of RPMI-1640 include: fusion protocols and growth of hybrid cells, culture of normal human leukocytes in monolayer and suspension, and support of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinoma growth (to name a few).
Storage Conditions: 2°C to 8°C
Shipping Condition: ambient temperature
Harmonization Code: 3821.00.0000
Max Shelf Life: 24 months