RPMI-1640 Medium without Phenol Red and L-Glutamine

ItemSize List Price Qty
112-040-101 500 mL $30.11


RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.

  • With 2.0 g/L sodium bicarbonate
  • Without HEPES buffer


RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.

Common applications of RPMI-1640 include: fusion protocols and growth of hybrid cells, culture of normal human leukocytes in monolayer and suspension, and support of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinoma growth (to name a few).


For those downstream protein purification studies, or other processes where the presence of phenol red is undesirable. Phenol red has been shown to interfere with the growth of some cells at clonal densities; use this medium when working with stem cells or when growing cell at low densities Phenol red may interfere with some chromatography processes involved with downstream purification.


Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.0 ± 0.2

Osmolality:  300 ± 5% mOsm/Kg H2O

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3821.00.0000


Max Shelf Life: 24 months


Additional information

Weight 32 oz
Dimensions 3 × 12 × 7 in
Vendor Number


Unit Quantity

500 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 112-040
Formulation 112-040