Description
SOC medium is a ready-to-use microbial growth medium, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli.
- Without magnesium chloride, needs to be added before use.
- Autoclave sterilized
Applications
SOC medium is used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
More specifically SOC Medium can be used in the propagation of DH-alpha (of E. coli strains) primarily in the recovery step of Escherichia coli competent cell transformations, and to maximize the transformation efficiency of competent cells.
SOC = Super Optimal broth with Catabolites repression
Recommendation
Immediately before use, add 2 M magnesium chloride to a final concentration of 20 mM (10 mL MgCl2/L).
Storage Conditions: 15℃ to 30℃
Shipping Condition: ambient temperature
Autoclave Sterilized
Max Shelf Life: 24 months from date of manufacture
Harmonization Code: 3822.00.5090
UNSPSC: 41106200
ECCN: EAR99
