SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli.
- Without magnesium chloride, needs to be added before use.
- Autoclave sterilized
SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
More specifically SOC Medium can be used: in the propagation of DH-alpha (of E. coli strains) primarily in the recovery step of Escherichia coli competent cell transformations, and to maximize the transformation efficiency of competent cells.
Immediately before use, add 2M magnesium chloride to a final concentration of 20mM (10ml MgCl2/L).
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
Harmonization Code: 3822.00.5090
Max Shelf Life: 24 months