Description
Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. In addition to its buffering capacity, it provides nutritional value in the culturing of mammalian cells.
- 7.5% w/v in water
- Endotoxin and Mycoplasma Tested
Synonyms: Sodium hydrogen carbonate, carbonic acid sodium salt (1:1), carbonic acid monosodium salt, sodium carbonate (1:1), sodium bicarbonate
Linear formula: NaHCO3
CAS No.: 144-55-8
Applications
Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells in culture. Sodium Bicarbonate, 7.5% is useful as a buffer for culturing a broad spectrum of mammalian cells.
Recommendation
Dilute Sodium Bicarbonate in appropriate cell culture media, to a final concentration of 5-15 mM
Storage Conditions: 15°C to 30°C
Shipping condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 290 ± 5% mOsM/kg H2O
Concentration: 0.893 mol/L
Max Shelf Life: 24 months from date of manufacture
Harmonization Code: 3822.00.5090
ECCN: EAR99