Description
TNE Buffer is often used as a base material to generate lysis buffers.
- Filtered through a 0.22 µm membrane
- DNase, RNase, and Protease tested
Applications
More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or reporter virus particles and the flurometric determination of DNA content.
Recommendation
Without correcting the pH, concentrated virus can be used in non-denaturing SDS/PAGE-Western Blot analysis.
If the pH is corrected to inhibit triggering of the viral fusion machinery (for West Nile Virus, this corresponds to pH 7.4), the concentrated virus will remain infectious.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
Sterile Filtered
pH: 8.0 ± 0.02
Harmonization Code: 3822.00.5090
ECCN: EAR99
Max Shelf Life: 36 months
