Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions.
Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids.
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1 M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
DNase RNase and Protease Tested
pH: 7.5± 0.1
Harmonization Code: 3822.00.5090
Max Shelf Life: 36 months