Tris HCl (2 M), pH 7.5

ItemSize List Price Qty
351-048-101 500 mL $68.30

Description

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions.

Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids.

Applications

Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).

Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1 M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.

 

Storage Conditions: 15°C to 30°C

Shipping Condition: ambient temperature

Sterile Filtered

DNase RNase and Protease Tested

pH: 7.5± 0.1

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3822.00.5090

ECCN: EAR99

Max Shelf Life: 36 months

Additional information

Weight 32 oz
Dimensions 3 × 12 × 7 in
Vendor Number

351-048-101

Unit Quantity

500 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 351-048