Trypsin (0.05%) – EDTA (0.1%)

ItemSize List Price Qty
118-087-721EA 100 mL $19.10

Description

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

  • With Phenol Red
  • Without Calcium and Magnesium
  • In PBS (Phosphate Buffered Saline)

Applications

Trypsin is a serine protease derived from porcine pancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.

Ethylenediaminetetraacetic acid (EDTA), a chelating agent often added to enhance enzymatic activity of trypsin solution. EDTA acts by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion.

Trypsin, 0.05% – EDTA, 0.1% assists in the dissociation of cell monolayers and tissue; most useful when dissociating fibroblasts and keratinocytes. While, Trypsin 0.25% – EDTA 0.02%, assists in detaching normal and transformed cells from vessel surfaces.

 

Storage Conditions: -10°C to -20°C

Shipping Condition: on dry ice*

Sterile Filtered

Performance Tested

Max Shelf Life: 24 months from date of manufacture

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3822.00.5090

ECCN: EAR99

*NOTE: During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not effect the functionality of the trypsin.

 

Additional information

Weight 128 oz
Dimensions 3 × 8 × 5 in
Vendor Number

118-087-721EA

Unit Quantity

100 mL

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 118-087
Technical Information Sheet 118-087