Trypsin 2.5%

ItemSizeList PriceQty
118-086-721 Pack 4 X 100 mL $172.13
118-086-721EA 100 mL $46.05


Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

  • Without EDTA


Trypsin is a serine protease derived from porcine pancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.


Do not add calcium or magnesium.
Prolonged exposure can negatively affect cell viability. Be aware of exposure time.


Storage Conditions: -10°C to -20°C

Shipping Condition: on dry ice*

Sterile Filtered

Performance Tested

Max Shelf Life: 24 months from date of manufacture

Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health

Harmonization Code: 3822.00.5090


*NOTE: During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not effect the functionality of the trypsin.

Additional information

Product Manufacturer

Quality Biological

Product Documentation

Safety Data Sheet 118-086
Technical Information Sheet 118-086